| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Zunongwangia profunda | TGCAAAACACGCCACCAC | TGGCACGTCCTCGATAACTG | 59.51 | 59.83 | 206 | 133 |
100.00%
|
100.00%
|
| Primer 2 | Zunongwangia profunda | TGCAAAACACGCCACCAC | CTGGGAAAATTGGCACGTCC | 59.51 | 59.76 | 216 | 133 |
100.00%
|
100.00%
|
| Primer 3 | Zunongwangia profunda | TGCAAAACACGCCACCAC | TGGGAAAATTGGCACGTCC | 59.51 | 58.65 | 215 | 133 |
100.00%
|
100.00%
|
| Primer 4 | Zunongwangia profunda | ACCTGGTACGCCATTGTGG | ACCGCTTGCAGCATTAGC | 60.00 | 58.81 | 272 | 133 |
100.00%
|
100.00%
|
| Primer 5 | Zunongwangia profunda | ACCTGGTACGCCATTGTGG | CACCGCTTGCAGCATTAGC | 60.00 | 60.23 | 273 | 133 |
100.00%
|
100.00%
|
| Primer 6 | Zunongwangia profunda | TGCAAAACACGCCACCAC | GCACGTCCTCGATAACTGC | 59.51 | 58.71 | 204 | 132 |
100.00%
|
100.00%
|
| Primer 7 | Zunongwangia profunda | CGCTAAAAGCTGAAGTGGAGC | TCAAATTGCGGTGCGGTG | 59.87 | 59.36 | 244 | 130 |
100.00%
|
100.00%
|
| Primer 8 | Zunongwangia profunda | GCTGAAGTGGAGCGAATTTGG | TCAAATTGCGGTGCGGTG | 60.14 | 59.36 | 236 | 130 |
100.00%
|
100.00%
|
| Primer 9 | Zunongwangia profunda | TGGGCAGACGGTAATGAAGG | TTCCTGGGCTGCTTTCAGG | 59.75 | 59.92 | 165 | 129 |
100.00%
|
100.00%
|
| Primer 10 | Zunongwangia profunda | ACACCGGTTTTGGGGTAGG | TCCCCTGGGGTTGTTTTGG | 59.54 | 59.77 | 170 | 125 |
100.00%
|
100.00%
|